Acute Meningitis : A Two Years Surveillance on Paediatric Population
(Sprache: Englisch)
Early and accurate diagnosis of bacterial meningitis remains a challenge to the clinician because of its rapid lethal course. The common practice of antibiotic therapy prior to lumbar puncture reduces the chance of isolation of bacteria in CSF culture,...
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Early and accurate diagnosis of bacterial meningitis remains a challenge to the clinician because of its rapid lethal course. The common practice of antibiotic therapy prior to lumbar puncture reduces the chance of isolation of bacteria in CSF culture, making the etiological diagnosis difficult. The present study was done to evaluate a multiplex PCR based method for simultaneous detection of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. Three species-specific primers were used along with universal primers of bacterial gene 16S rRNA, in a two-stage PCR assay. A cross sectional prospective study was carried out with 140 children (2 months to 12 years of age) with clinical suspicion of meningitis. Of these 140 patients, 42 (30%) cases were diagnosed as bacterial meningitis and other 98 (70%) as viral meningitis. Among the antibiotic users, bacterial meningitis case detection by PCR was higher (65.52%) than that of culture (10.34%) and Gram staining (6.90%). Regarding the advantages and disadvantages of the conventional bacteriological methods and molecular biology techniques, such as PCR, it is recommended to use both the methods in clinical practice.
Autoren-Porträt von Nilufar Yeasmin Nili
Nili, Nilufar YeasminDr. Nilufar Yeasmin Nili, a Physician, Lecturer, Clinical Microbiologist & Population Scientist, was born in 02-02-1980 in Dhaka, Bangladesh. She completed MBBS, MPS, FCGP in Family Medicine CCD in Diabetology, CCCD in Cardiology & M.Phil in Medical Microbiology. Now she is working as a Medical Officer in Dept.of Surgery in a Reputed Govt.Hospital.
Bibliographische Angaben
- Autor: Nilufar Yeasmin Nili
- 2019, 152 Seiten, Maße: 22 cm, Kartoniert (TB), Englisch
- Verlag: LAP Lambert Academic Publishing
- ISBN-10: 6200079099
- ISBN-13: 9786200079091
Sprache:
Englisch
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