Advances and Clinical Practice in Pyrosequencing

Preparation of single-stranded DNA for pyrosequencing by LATE-PCR.- A simplified protocol for preparing pyrosequencing templates based on LATE-PCR using whole blood as starting material directly.- Improvement of LATE-PCR to prepare pyrosequencing template.- Pyrosequencing-templates generated by nicking PCR products with nicking endonuclease.- Pyrosequencing-templates generated by asymmetric Nucleic Acid Sequence-Based Amplification (asymmetric-NASBA).- Gel immobilization of acrylamide-modified single-stranded DNA template for pyrosequencing.- Multiplex PCR based on a universal biotinylated primer to generate templates for pyrosequencing.- A novel pyrosequencing principle based on AMP-PPDK reaction for improving the detection limit.- Pyrosequencing chemistry coupled with modified primer extension reactions for quantitative detection of allele frequency in a pooled sample.- A gel-free SNP genotyping method based on pyrosequencing chemistry coupled with modified primer extension reactionsdirectly from double-stranded PCR products.- Improvement of pyrosequencing to allow multiplex SNP typing in a program.- Improvement of pyrosequencing-sensitivity based on the capture of free adenosine 5'-phosphosulfate with adenosine triphosphate sulfurylase.- Using polymerase preference index to design imLATE-PCR primers for an efficient pyrosequencing.- Preparation of thermalstable biotinlyted firefly luciferase and its application in pyrosequencing.- Expression of thermostable recombinant luciola lateralis luciferase and its function on setting up of heat-stable pyrosequencing system.- Expression and purification of ATP sulfurylase from saccharomyces cerevisias in Escherichia coli and its application in pyrosequencing.- Characterization of recombinant Escherichia coli single-strand binding protein and its application in pyrosequencing.- Expression of PPDK from Microbispora Rosea Subsp.aerata in E.coli and its application in pyrosequencing.- Construction of 3-plex barcodes for