Ancient DNA

Setting Up an Ancient DNA Laboratory.- Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate.- Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step.- Extraction of Highly Degraded DNA from Ancient Bones and Teeth.- Sampling and Extraction of Ancient DNA from Sediments.- Extraction of Ancient DNA from Plant Remains.- DNA Extraction from Keratin and Chitin.- Double-Stranded Library Preparation for Ancient and Other Degraded Samples.- A Method for Single-Stranded Ancient DNA Library Preparation.- Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms.- Whole-Genome Capture of Ancient DNA Using Homemade Baits.- Generating RNA Baits for Capture-Based Enrichment.- Hybridization Capture of Ancient DNA Using RNA Baits.- Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA.- Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis.- Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA.- Authentication and Assessment of Contamination in Ancient DNA.- Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence.