Antibiotics

Antibiotics - Precious Goods in Changing Times.- Mining Bacterial Genomes for Secondary Metabolite Gene Clusters.- Production of Antimicrobial Compounds by Fermentation.- Structure Elucidation of Antibiotics by NMR Spectroscopy.- Computer-Aided Drug Design Methods.- Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents.- Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds.- Determination of Bacterial Membrane Impairment by Antimicrobial Agents.- Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes.- Measurement of Cell Membrane Fluidity by Laurdan GP:Fluorescence Spectroscopy and Microscopy.- In vitro Assays to Identify Antibiotics Targeting DNA Metabolism.- Fluorescence-based real-time Activity Assays to Identify RNase P inhibitors.- Reporter Gene-based Screening for TPP Riboswitch Activators.- Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation.-Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen.- Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory Evolution.- Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research.- Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic Research.- Functional Metagenomics to Study Antibiotic Resistance.- Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing.