Bacterial Cell Surfaces

A Bird's Eye View of the Bacterial Landscape.- Visualizing the Bacterial Cell Surface: An Overview.- Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems.- Experimental Manipulation of the Microbial Functional Amyloid Called Curli.- Visualization of Gram-Positive Bacterial Pili.- Single Cell Microfluidic Studies of Bacterial Motility.- Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule.- Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy.- Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance.- Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System.- Pore Formation by T3SS Translocators: Liposome Leakage Assay.- Isolation of Bacterial Type IV Machine Subassemblies.- Production and Crystallization of Bacterial Type V Secretion Proteins.- Assembly of Bacterial Outer Membrane Proteins.- The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and Characterization.- Quantitative and Qualitative Preparations of Bacterial Outer Membrane Vesicles.- In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate.- Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes.- Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis.- Protein Disulfide Bond Formation in the Periplasm: Determination of the In Vivo Redox State of Cysteine Residues.- Using Reporter Genes and the Escherichia coli ASKA Over-Expression Library in Screens for Regulators of the Gram Negative Envelope Stress Response.- Isolation of Bacteria Envelope Proteins.- Patch Clamp Electrophysiology for the Study of Bacterial Ion Channels inGiant Spheroplasts of E. coli.- Electrophysiological Characterization of Bacterial Pore-Forming Proteins in Planar Lipid Bilayers.