Bacterial Transcriptional Control

Mapping the Escherichia coli Transcription Elongation Complex with Exonuclease III.- Purification of Bacterial RNA Polymerase: Tools and Protocols.- Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues.- In vitro and in vivo Methodologies for Studying the Sigma54-dependent Transcription.- Methods for the Assembly and Analysis of in vitro Transcription-coupled-to-translation Systems.- Site-specific Incorporation of Probes into RNA Polymerase by Unnatural-amino-acid Mutagenesis and Staudinger-Bertozzi Ligation.- Reconstitution of Factor-dependent, Promoter Proximal Pausing in Drosophila Nuclear Extracts.- Direct Competition Assay for Transcription Fidelity.- Single-stranded DNA Aptamers for Functional Probing of Bacterial RNA Polymerase.- Biochemical Analysis of Transcription Termination by RNA Polymerase III from Yeast Saccharomyces cerevisiae.- Use of RNA Polymerase Molecular Beacon Assay to Measure RNA Polymerase Interactions with Model Promoter Fragments.- Preparation of cDNA Libraries for High-throughput RNA Sequencing Analysis of RNA 5' Ends.- In situ Footprinting of E. coli Transcription Elongation Complex with Chloroacetaldehyde.- Using Solutes and Kinetics to Probe Large Conformational Changes in the Steps of Transcription Initiation.- Manipulating Archaeal Systems to Permit Analyses of Transcription Elongation-termination Decisions in vitro.- Purification of Active RNA Polymerase I from Yeast.- Transcription in Archaea: preparation of Methanocaldococcus jannaschii Transcription Machinery.- Transcription in Archaea: in vitro Transcription Assays for mjRNAP.- Experimental Analysis of hFACT Action during Pol II Transcription in vitro.- ChIP-seq for Genome-scale Analysis of Bacterial DNA-binding Proteins.

Name: Irina Artsimovitch Address: Department of Microbiology The Ohio State University 484 West 12th Avenue Columbus, OH 43210 Phone: (614) 292-6777 Email: artsimovitch.1@osu.edu