Cellulases

Cellulases: Role in Lignocellulosic Biomass Utilization.- Discovery of a Novel Fungus with an Extraordinary b-Glucosidase.- Discovery of New Cellulases Using Classical or Molecular Methods.- Isolation and Screening of Cellulolytic Filamentous Fungi.- Isolation and Screening of Cellulose Degrading Microorganisms from Different Ecological Niches.- Isolation of Cellulolytic Bacteria from the Rumen.- Methods for Discovery of Novel Cellulosomal Cellulases using Genomics and Biochemical Tools.- Metatranscriptomic Techniques for Identifying Cellulases in Termites and Their Symbionts.- Discovery of Novel Cellulases Using Proteomic Strategies.- Identification of Key Components for the Optimization of Cellulase Mixtures Using a Proteomic Strategy.- Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger.- Advanced Cloning Tools for Construction of Designer Cellulosomes.- Evaluation of Thermal Stability of Cellulosomal Hydrolases and Their complex Formation.- Endoglucanase (EG) Activity Assays.- Cellobiohydolase (CBH) Activity Assays.- Two-Dimensional High-Throughput Endo-Enzyme Screening Assays Based on Chromogenic Polysaccharide Hydrogel and Complex Biomass Substrates.- Analytical Tools for Characterizing Cellulose-Active Lytic Polysaccharide Monooxygenases (LPMOs).- Light Induced Electron Transfer Protocol for Enzymatic Oxidation of Polysaccharide.- Purification of Cellulase from Cellulolytic Microorganisms and Characterization of the Total Cellulase Activities (TCA).- Fungal On-Site Production of Cellulolytic Enzymes by the Sequential Cultivation Method.- Test of Efficacy of Cellulases for Biomass Degradation.- Homology Modeling for Enzyme Design.