Chemical Cytometry

PrefaceORIGIN, CURRENT STATUS, AND FUTURE PERSPECTIVES OF CHEMICAL CYTOMETRYThe Cell and CytometryFlow and Image CytometryChemical CytometryChemical Cytometry of DNA and mRNAMetabolic CytometryFuture Perspectives on Instrumentation for Chemical CytometryMETABOLIC CYTOMETRY - THE STUDY OF GLYCOSPHINGOLIPID METABOLISM IN SINGLE PRIMARY CELLS OF THE DORSAL ROOT GANGLIAIntroductionMaterial and MethodsResults and DiscussionConclusionsCELL SIGNALING STUDIES AT THE SINGLE-CELL LEVELIntroductionAnalytes Examined and Reporters UsedCell Preparation and Reporter LoadingCell Lysis and Sampling TechniquesElectrophoresis Separation ConditionsDetectionAutomation and ThroughputULTRASENSITIVE DETECTION OF LOW-COPY-NUMBER MOLECULES FROM SINGLE CELLSIntroductionMicrochip Designs for Single-Cell Analysis and/or Cell ManipulationUltrasensitive Detection Methods for Single-Cell AnalysisSingle-Cell Analysis with Single-Molecule Sensitivity on Integrated Microfluidic ChipConclusionsCAPILLARY ELECTROPHORESIS OF NUCLEIC ACIDS AT THE SINGLE-CELL LEVELIntroductionOn-Line Cell AnalysisDirect Gene and Gene Expression Analysis Without AmplificationPotential Alternative Techniques for Single-Cell Gene and Gene Expression AnalysisConclusionsMICROFLUIDIC TECHNOLOGY FOR SINGLE-CELL ANALYSISIntroductionBiological Significance of Single-Cell AnalysisMicrofluidic Devices in Our LaboratoriesMaterials, Methods, and ProtocolsConclusionsON-CHIP ELECTROPORATION AND ELECTROFUSION FOR SINGLE-CELL ENGINEERINGIntroductionSingle-Cell Electroporation in Microfluidic DevicesSingle-Cell Electrofusion in Microfluidic DevicesConclusionsELECTROPORATIVE FLOW CYTOMETRY FOR SINGLE-CELL ANALYSISIntroductionFlow-Through Electroporation under Constant VoltageElectroporative Flow Cytometry for Detecting Protein TranslocationElectroporative Flow Cytometry for Measuring Single-Cell BiomechanicsElectroporative Flow Cytometry for Selectively Releasing and Analyzing Specific Intracellular MoleculesConclusionULTRASENSITIVE ANALYSIS

Chang Lu is an associate professor of chemical engineering at Virginia Tech, Blacksburg (USA). From 2004 to 2009 he was an assistant and associate professor of biological engineering at Purdue University at West Lafayette. He obtained his academic degrees from Peking University and from the University of Illinois at Urbana-Champaign, and received post-doctoral training at Cornell University. His research has been focused on Microfluidic devices for single cell analysis and tissue engineering. Professor Lu has received a number of awards, including the NSF Career Award and the Wallace H. Coulter foundation Early Career Award in Biomedical Engineering.