Chromosome and Genomic Engineering in Plants

Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences.- Method for Biolistic Site-Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase.- Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking.- Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants.- One-Step Generation of Chromosomal Rearrangements in Rice.- Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana.- Gametocidal Systems for Dissecting Wheat Chromosomes.- CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases.- Targeted Mutagenesis in Rice Using and TALENs and the CRISPR/Cas9 System.- Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination.- Development of Genome Engineering Tools fromPlant-Specific PPR Proteins Using Animal Cultured Cells.- Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes.- Image Analysis of DNA Fiber and Nucleus in Plants.- Detection of Transgenes on DNA Fibers.- Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging Systems.- Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes.- Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements.