Ebolaviruses

Marburg and Ebola Viruses: A Look Back and Lessons for the Future.- 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies.- Ebolavirus: An Overview of Molecular and Clinical Pathogenesis.- Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms.- Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins.- Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems.- Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation.- Modeling Ebolavirus Budding with Virus-Like Particles.- Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays.- Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins.- Non-Radioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA.- A Semi-Automated High Throughput Microtitration Assay for Filoviruses.- Generation of Recombinant Ebola Viruses Using Reverse Genetics.- Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals.- Live-Cell Imaging of Filoviruses.- Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds.- Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence.- Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells.- Electron Microscopy of Ebola Virus-Infected Cells.- Validating the Inactivation Effectiveness of Chemicals on Ebola Virus.- Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS).- Assessing Antiviral Countermeasures Using Mouse Models of Ebolavirus Infection.- Evaluation of Ebola Virus Countermeasures in Guinea Pigs.- Evaluation of Medical Countermeasures against Ebolaviruses in Non-Human Primate Models.- Quantification of Filovirus Glycoprotein-Specific Antibodies.-

"This book comprises three review chapters on filoviruses and 27 protocol chapters. ... Scientists working on filoviruses and those wishing to enter the field will find a compendium of carefully written methods and protocols relevant to their work. ... What makes the book stand out from many other volumes in this series is the inclusion of the three review chapters, which by themselves earn the book a spot on the bookshelf of any scientist working in the field." (Christoph Wirblich, Doody's Book Reviews, January, 2018)