Flavins and Flavoproteins

Selection of Riboflavin Overproducing Strains of Lactic Acid Bacteria and Riboflavin Direct Quantification by Fluorescence.- Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida Famata.- Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida Famata.- Functional Study of the Human Riboflavin Transporter 2 Using Proteoliposomes System.- Heterologous Over-Expression of Human FAD Synthase Isoforms 1 and 2.- Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD / Protein Monomer Ratio by UV/Vis Spectra.- Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions.- Redox Titration of Flavoproteins: An Overview.- Anaerobic Stopped-Flow Spectrophotometry with Photodiode Array Detection in the Pre-Steady State: An Application to Elucidate Oxido-Reduction Mechanisms in Flavoproteins.- Atomic Force Microscopy: Single Molecule Imaging and Force Spectroscopy in the Study of Flavoproteins Ligand Binding and Reaction Mechanisms.- Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State.- Ligand Binding in Allosteric Flavoproteins: Part 2. Quantitative Analysis of the Redox-Dependent Interaction of the Apoptosis-Inducing Factor (AIF) with Its Protein Partner.- Using D- and L-Amino Acid Oxidases to Generate the Imino Acid Substrate to Measure the Activity of the Novel Rid (Enamine/Imine Deaminase) Class of Enzymes.- The In Vitro Production of prFMN for Reconstitution of UbiD Enzymes.- Alcohol Oxidase from the Methylotrophic Yeast Ogataea polymorpha: Isolation, Purification, and Bioanalytical Application.- Flavocytochrome b2 of the Methylotrophic Yeast Ogataea polymorpha:Construction of Overproducers, Purification, and