Kidney Organogenesis

Preface... Table of Contents... Contributing Authors... Part I Generation of Mosaic Embryonic Kidneys and Culture of the Kidney Tissues Ex Vivo 1. Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development Frank Costantini 2 Mouse Ex Vivo Kidney Culture Methods Anneliis Ihermann-Hella and Satu Kuure 3 Serum-Free Organ Culture of the Embryonic Mouse Ureter Filipa M. Lopes and Adrian S. Woolf 4 Experimental Tubulogenesis Induction Model in the Mouse Aleksandra Rak-Raszewska Part II Embryonic Kidney Progenitor Cell and 3D Kidney Model Cell Like Based Cultures 5 Dissociation of Tissues for Single Cell Analysis Andrew S. Potter and S. Steven Potter 6 Long Term Culture of Nephron Progenitor Cells Ex Vivo Aaron C. Brown, Ashwani K. Gupta, and Leif Oxburgh 7 3D Cell Culture Models of Epithelial Tissues Kai Zhang and Aki Manninen Part III Programming the Kidney Cell Fates in Human Pluripotent Cells 8 Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells Yasuhiro Yoshimura, Atsuhiro Taguchi, and Ryuichi Nishinakamura 9 Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells Krithika Hariharan, Petra Reinke, and Andreas Kurtz 10 Protocol to Generate Ureteric Bud Structures from Human iPS Cells Shin-Ichi Mae, Makoto Ryosaka, and Kenji Osafune Part IV Protocols to Target the Functional Players in Kidney Ontogenesis 11 Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture Melanie L. Lawrence, Mona Elhendawi, and Jamie A. Davies 12 Virus as Renal Functional Genetic Tools Abhishek Sharma and Sandhanakrishnan Cattavarayane 13 Gene-Editing in 3D Cultured Nephron Progenitor Cell Lines Zhongwei Li, Toshikazu Araoka, and Juan Carlos Izpisua Belmonte Part V 4D and 3D Ex Vivo Imaging of Embryonic Kidney Decellularization 14 Time-Lapse Technologies and 4D Imaging of Kidney Development Ulla Saarela and Ilya Skovorodkin 15 Decellularizing and Recellularizing Adult