Membrane Biogenesis

Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry.- Thin-Layer Chromatography of Phospholipids.- Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics.- Using 2D Crystals to Analyze the Structure of Membrane Proteins.- Crystallization of Membrane Proteins.- Molecular Dynamics Simulations of Membrane Proteins.- Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins.- Topology Determination of Untagged Membrane Proteins.- self-assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses.- The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane.- Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance.- Peptide Interaction with and Insertion into Membranes.- Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems.- Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast.- Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins.- Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry.- Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria.- Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells.- An Assay to Monitor the Membrane Integration of Single-Span Proteins.- Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria.- Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes.- Single Channel Analysis of MembraneProteins in Artificial Bilayer Membranes.- Quantification of Protein Complexes by Blue Native Electrophoresis.- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions.