Metabolic Signaling

A Protocol to Compare Methods for Untargeted Metabolomics.- High Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry.- Measuring the Nutrient Metabolism of Adherent Cells in Culture.- 13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells.- Measuring In Vivo Tissue Metabolism using 13C Glucose Infusions in Mice.- Measuring Human Lipid Metabolism using Deuterium Labeling: In vivo and in vitro.- Measuring Rates of ATP Synthesis.- Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay.- Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells using Radioactive Tracers.- Determining Compartment Specific Metabolic Fluxes.- Determining the Impact of Metabolic Nutrients on Autophagy.- Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism.- Determining Macrophage Polarization upon Metabolic Perturbation.- Assessing the Impact of the Nutrient Microenvironment on The Metabolism of Effector CD8+ T Cells.- Development of Patient-derived Tumor Xenograft Models.- Imaging Glioma Progression by Intravital Microscopy.- Lipectomizing Mice for Applications in Metabolism.- Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants.- Determining the Intracellular Organization of Organelles.- The Fundamentals of Constructing and Interpreting Heat Maps.