Metabolomics in Practice

PrefaceTHE SAMPLING AND SAMPLE PREPARATION PROBLEM IN MICROBIAL METABOLOMICSIntroductionMicroorganisms and Their PropertiesSampling MethodsQuenchingMetabolite ExtractionApplication of 13C-Labeled Internal StandardsConclusionsTANDEM MASS SPECTROMETRY HYPHENATED WITH HPLC AND UHPLC FOR TARGETED METABOLOMICSIntroductionLC-MS-Based Targeted MetabolomicsLiquid ChromatographyMass SpectrometrySample PreparationRelative and Absolute QuantificationApplicationsSynopsisUNCERTAINTY OF MEASUREMENT IN QUANTITATIVE METABOLOMICSIntroductionUncertainties of Quantitative MS ExperimentsConcluding RemarksGAS CHROMATOGRAPHY AND COMPREHENSIVE TWO-DIMENSIONAL GAS CHROMATOGRAPHY HYPHENATED WITH MASS SPECTROMETRY FOR TARGETED AND NONTARGETED METABOLOMICSIntroduction and ScopeSample Preparation for GC-Based Metabolite ProfilingGC-MS and GC X GC-TOFMS Instrumentation for MetabolomicsData Analysis Strategies and SoftwareIllustrative Examples and Concluding RemarksLC-MS-BASED NONTARGETED METABOLOMICSIntroductionLC-MS-Based Untargeted MetabolomicsStudy DesignSample PreparationAnalytical StrategiesData AnalysisMetabolite IdentificationApplicationsSynopsisTHE POTENTIAL OF ULTRAHIGH RESOLUTION MS (FTICR-MS) IN METABOLOMICSIntroductionMetabolomics TechnologiesPrinciples of FTICR-MSProceeding in MetabolomicsApplication Example in Metabolomics Using FTICR-MS Exhaled Breath CondensateConclusion and RemarksNMR-BASED METABOLOMICS ANALYSISIntroductionPlatforms for MetabolomicsNMR for MetabolomicsApplications of NMR-Based MetabolomicsFuture Prospects and ConclusionsPOTENTIAL OF MICROFLUIDICS AND SINGLE CELL ANALYSIS IN METABOLOMICS (MICROMETABOLOMICS)IntroductionSample Processing for MetabolomicsMicrofluidic Separations for Metabolic AnalysisMicrofluidics for Cellular AnalysisA Look ForwardDATA PROCESSING IN METABOLOMICSIntroduction and ScopeCharacteristics of Metabolomics DataTypes of Biological Questions AskedValidationOverview of MethodsMETABOLIC FLUX ANALYSISIntroductionPrerequisites for Flux

Michael Lämmerhofer is Associate Professor at the Department of Analytical Chemistry of the University of Vienna (Austria). Having obtained his academic degrees from the University of Graz, he spent two years as a post-doctoral fellow at the Department of Chemistry of the University of California, Berkeley (USA). His current research is directed towards the targeted quantitative analysis of extracellular and intracellular metabolites, mainly with focus on applications in industrial biotechnology.

Wolfram Weckwerth is Full Professor and Head of the Department of Molecular Systems Biology at the University of Vienna (Austria). Having obtained his academic degrees from the Technical University of Berlin (Germany), he moved to the Max-Planck-Institute of Molecular Plant Physiology, where he pioneered metabolomic methods and integrated proteomic and computer-based approaches into a systems biology framework. After his habilitation in Systems Biology at the University of Potsdam, he took over a position as a head of a research lab in the German FORSYS systems biology initiative, before founding the Department of Molecular Systems Biology at the University of Vienna in 2008.