Molecular Chaperones

Targeted Deletion of Hsf1, 2, and 4 Genes in Mice.- The Role Of Heat Shock Factors in Stress-Induced Transcription.- Hsp90 and Client Protein Maturation.- The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function.- Detecting Hsp90 Phosphorylation.- Role of Molecular Chaperones in Biogenesis of the Protein Kinome.- Nucleotide Exchange Factors for Hsp70 Chaperones.- Reconstitution of CHIP E3 Ubiquitin Ligase Activity.- Structure-Functions of HspB1 (Hsp27).- Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene.- Quantification of HSP27 and HSP70 Molecular Chaperone Activities.- Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA.- Analysis of Heat Shock Protein Localization Using Flow Cytometry.- Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry.- Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks.- Hsp70: Anti-Apoptotic and Tumorigenic Protein.- Determination of Cell Survival or Death.- Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer.- Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells.- Isolation of Heat Shock Protein Complexes.- Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein.- Investigating Receptors for Extracellular Heat Shock Proteins.- Analysis of Cellular Migration Using a Two-Chamber Methodology.

From the reviews:"This book details current methods and protocols essential for understanding the biological properties and roles of the molecular chaperones ... . It appears to be written for all scientists interested in understanding the roles of molecular chaperones, which would include students and researchers interested in heat shock proteins. ... This will be a useful guide for all scientists interested in the field of heat shock proteins and molecular chaperones and their role in health and disease." (Omer Iqbal, Doody's Book Reviews, December, 2011)