Multiprotein Complexes

Production of Multi-Subunit Membrane Protein Complexes.- Production of Multiprotein Complexes using the Baculovirus Expression System: Homology Based and Restriction Free Cloning Strategies for Construct Design.- Tagging Proteins with Fluorescent Reporters using the CRISPR/Cas9 System and Double Stranded DNA Donors.- Validation of the Production of Antibodies in Different Formats in the HEK 293 Transient Gene Expression System.- The Intervening Removable Affinity Tag (iRAT) System for the Production of Recombinant Antibody Fragments.- Isolation of Artificial Binding Proteins (Affimer Reagents) for Use in Molecular and Cellular Biology.- Interactions Measurements of Protein-DNA Complexes by Isothermal Titration Calorimetry (ITC) and Microscale Thermophoresis (MST).- switchSENSE Technology for Analysis of DNA Polymerase Kinetics.- Sedimentation Velocity Methods for Characterization of Protein Heterogeneity and Protein Affinity Interactions.- Hands on Native Mass Spectrometry Analysis of Multi-Protein Complexes.- Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry.- Integrative Mass Spectrometry-Based Approaches for Modeling Macromolecular Assemblies.- Optimization of Sample Preparation for the Observation of Macromolecular Complexes by Electron (cryo-)Microscopy.- Solubilization and Stabilization of Native Membrane Proteins for Drug Discovery.- Practical Aspects of Super-Resolution Imaging and Segmentation of Macromolecular Complexes by dSTORM.- Multicolor FRET-FLIM Microscopy to Analyze Multiprotein Interactions in Live Cells.- Context-Specific and Proximity-Dependent Labeling for the Proteomic Analysis of Spatiotemporally Defined Protein Complexes with Split-BioID.- A Directed Evolution System for Lysine Deacetylases.