p53 Protocols

Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues.- Detecting p53 Isoforms at Protein Level.- Autophagy, Senescence and Apoptosis.- p53 and Cell Cycle Effects after DNA Damage.- p53 Ubiquitination and Proteasomal Degradation.-Identification of p53 in Mitochondria.-Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis.-Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization.- Determine the Effect of p53 on Chemosensitivity.-Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells.- Mutant p53 in Cell Adhesion and Motility.- Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication.- Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts.- p53 Actions on MicroRNA Expression and Maturation Pathway.- Isolation and Characterization of Murine Multipotent Lung Stem Cells.- Generation of p53 Knock-down Cell Lines.-ChIP for Identification of p53 Responsive DNA Promoters.- ChIP-on-chip to Identify Mutant p53 Targets.- ChIP Sequencing to Identify p53 Targets.- The p53-Mdm2 Loop: A Critical Juncture of Stress Response.