Pancreatic Cancer

Identification and Analysis of Precursors to Invasive Pancreatic Cancer.- Tissue Microarrays: Construction and Use.- Immunohistochemistry of Pancreatic Neoplasia.- A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer.- Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications.- Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis.- DNA Methylation Analysis in Human Cancer.- Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients.- Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine.- The Prevention and Genetics of Pancreatic Cancer - A Programmatic Approach.- Development of Orthotopic Pancreatic Tumor Mouse Models.- Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems.- Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound.- Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells.- Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells.- Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis.- Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo.- The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells.- Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods.- Evaluating Tumor Angiogenesis.- Pooled Genome-wide RNAi Screenings.- Pooled ShRNA screenings: Computational Analysis.

From the reviews of the second edition:"This book describes methods and techniques that are used in pancreatic cancer research. ... It will be most useful for postdoctoral researchers and novice principal investigators, as well as any investigators who want to focus on pancreatic cancer research, particularly if they are looking for a technique to fit a specific need. ... It is a useful reference and go-to guide for exploring new protocols and techniques. It is also handy for seeing how an existing technique might be altered slightly to suit another need." (Naomi Akagi, Doody's Book Reviews, September, 2013)