Plant Lipid Signaling Protocols
(Sprache: Englisch)
This book presents timely and important tools needed to elucidate particular roles of phosphoinositides in plants. It includes easy-to-use, reproducible protocols and contains expert tips on troubleshooting and avoiding known pitfalls.
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Produktinformationen zu „Plant Lipid Signaling Protocols “
This book presents timely and important tools needed to elucidate particular roles of phosphoinositides in plants. It includes easy-to-use, reproducible protocols and contains expert tips on troubleshooting and avoiding known pitfalls.
Klappentext zu „Plant Lipid Signaling Protocols “
As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.
Inhaltsverzeichnis zu „Plant Lipid Signaling Protocols “
Part I: Lipid Analyses1. Analyzing Plant Signaling Phospholipids through 32Pi-Labeling and TLC
Teun Munnik & Xavier Zarza
2. Analysis of D3-,4-,5-phosphorylated Phosphoinositides using HPLC
Teun Munnik
3. Mass Measurement of Polyphosphoinositides by Thin Layer- and Gas Chromatography
Mareike Heilmann and Ingo Heilmann
4. Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay
Ingo Heilmann and Imara Y. Perera
5. Quantification of Diacylglycerol by Mass Spectrometry
Katharina vom Dorp, Isabel Dombrink and Peter Dörmann
6. Distinguishing Phosphatidic Acid Pools from de novo Synthesis, PLD and DGK
Steven A. Arisz & Teun Munnik
7. Use of Phospholipase A2 for the Production of Lysophospholipids
Steven A. Arisz & Teun Munnik
8. Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas Chromatography
Vera Wewer, Peter Dörmann & Georg Hölzl
9. Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry
Sunitha Shiva, Hieu Sy Vu, Mary R. Roth, Zhenguo Zhou, Shantan Reddy Marepally, Daya Sagar Nune, Gerald H. Lushington, Mahesh Visvanathan, and Ruth Welti
10. Detection and Quantification of Plant Sphingolipids by LC-MS
Jonathan E. Markham
11. Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid
Nadja Stingl, Markus Krischke, Agnes Fekete and Martin J. Mueller
12. Analysis of Fatty Acid Amide Hydrolase (FAAH) Activity in Plants Sang-Chul Kim, Lionel Faure, and Kent D. Chapman
13. Ionization Behavior of
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Polyphosphoinositides Determined via the Preparation of pH Titration Curves using Solid State 31P NMR
Zachary T. Graber, and Edgar E. Kooijman
Part II: Enzyme Activities
14. Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant Peptides
Sylvie Collin and Françoise Cochet
15. Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase Assays
Yang Ju Im, Irena Brglez, Catherine Dieck, Imara Perera and Wendy F. Boss
16. Assaying Inositol and Phosphoinositide Phosphatase Enzymes
Janet L. Donahue, Mustafa Ercetin and Glenda E. Gillaspy
17. Determination of Phospholipase C Activity In Vitro
S.M. Teresa Hernández-Sotomayor and J. Armando Muñoz-Sanchez
18. Assaying Non-Specific Phospholipase C (NPC) Activity
Premysl Pejchar, Günther F. E. Scherer and Jan Martinec
19. Assaying Different Types of Plant Phospholipase D Activities In Vitro
Kirk L. Pappan and Xuemin Wang
20. Measuring PLD Activity In Vivo
Teun Munnik & Ana M. Laxalt
21. Assaying Plant Phosphatidic Acid Phosphatase Activity
Yuki Nakamura
22. Assay of Phospholipase A Activity
Michael Heinze and Werner Roos
Jonathan E. Markham
Part III: Protein-Lipid Intractions
23. Lipid-Binding Analysis using a Fat Blot Assay
Teun Munnik and Magdalena Wierzchowiecka
24. Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions
Magdalena M. Julkowska, Johanna M. Rankenberg and Christa Testerink
25. Lipid affinity beads - From Identifying New Lipid Binding Proteins to Assessing their Binding Properties
Fionn McLoughlin & Christa Testerink
Part IV:Imaging
26. Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants
Joop E.M. Vermeer and Teun Munnik
27. Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy
Sarah J. Swanson and Simon Gilroy
Zachary T. Graber, and Edgar E. Kooijman
Part II: Enzyme Activities
14. Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant Peptides
Sylvie Collin and Françoise Cochet
15. Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase Assays
Yang Ju Im, Irena Brglez, Catherine Dieck, Imara Perera and Wendy F. Boss
16. Assaying Inositol and Phosphoinositide Phosphatase Enzymes
Janet L. Donahue, Mustafa Ercetin and Glenda E. Gillaspy
17. Determination of Phospholipase C Activity In Vitro
S.M. Teresa Hernández-Sotomayor and J. Armando Muñoz-Sanchez
18. Assaying Non-Specific Phospholipase C (NPC) Activity
Premysl Pejchar, Günther F. E. Scherer and Jan Martinec
19. Assaying Different Types of Plant Phospholipase D Activities In Vitro
Kirk L. Pappan and Xuemin Wang
20. Measuring PLD Activity In Vivo
Teun Munnik & Ana M. Laxalt
21. Assaying Plant Phosphatidic Acid Phosphatase Activity
Yuki Nakamura
22. Assay of Phospholipase A Activity
Michael Heinze and Werner Roos
Jonathan E. Markham
Part III: Protein-Lipid Intractions
23. Lipid-Binding Analysis using a Fat Blot Assay
Teun Munnik and Magdalena Wierzchowiecka
24. Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions
Magdalena M. Julkowska, Johanna M. Rankenberg and Christa Testerink
25. Lipid affinity beads - From Identifying New Lipid Binding Proteins to Assessing their Binding Properties
Fionn McLoughlin & Christa Testerink
Part IV:Imaging
26. Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants
Joop E.M. Vermeer and Teun Munnik
27. Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy
Sarah J. Swanson and Simon Gilroy
... weniger
Bibliographische Angaben
- 2013, 1st ed., XI, 305 Seiten, 15 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Teun Munnik, Ingo Heilmann
- Verlag: Springer, Berlin
- ISBN-10: 1627034005
- ISBN-13: 9781627034005
- Erscheinungsdatum: 17.05.2013
Sprache:
Englisch
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