Polyadenylation in Plants
Methods and Protocols
(Sprache: Englisch)
This volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. numerous molecular, biochemical, and methods used to...
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This volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. numerous molecular, biochemical, and methods used to characterize polyadenylation sites on a genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Some are specific for plant research, but most can be adopted for research in other organisms.Authoritative and practical, Polyadenylation in Plants: Methods and Protocols provides scientists with a wide range of methods to study mRNA 3'-end formation in plants.
Inhaltsverzeichnis zu „Polyadenylation in Plants “
Part I: Bioinformatics Studies of Plant Polyadenylation1. Computational Analysis Of Plant Polyadenylation Signals
Xiaohui Wu, Guoli Ji, and Qingshun Quinn Li
2. Prediction of Plant mRNA Polyadenylation Sites
Xiaohui Wu, Guoli Ji, and Qingshun Quinn Li
3. Extraction of poly(A) Sites from Large-scale RNA-seq Data Min Dong, Guoli Ji, Qingshun Q. Li, and Chun Liang
4. Poly(A)-tag Deep Sequencing Data Processing To Extract poly(A) Sites
Xiaohui Wu, Guoli Ji, and Qingshun Quinn Li
5. Analysis of poly(A) Site Choice Using A Java-Based Clustering Algorithm
Patrick E. Thomas
6. RADPRE: a Computational Program for Identification of Differential mRNA Processing Including Alternative Polyadenylation
Denghui Xing and Qingshun Quinn Li
Part II: Biochemistry of Polyadenylation in Plants
7. Characterization Of Plant Polyadenylation Complexes By Using Tandem Affinity Purification
Hongwei Zhao?Xinfu Ye, and Qingshun Quinn Li
8. In vitro Analysis Of Cleavage And Polyadenylation In Arabidopsis
Hongwei Zhao and Qingshun Quinn Li
9. Production, Purification, and Assay of Recombinant Proteins for in vitro Biochemical Analyses of the Plant Polyadenylation Complex
Stephen A. Bell and Balasubrahmanyam Addepalli
10. Detection of Disulfide Linkage By Chemical Derivatization And Mass Spectrometry
Balasubrahmanyam Addepalli
11. Transient Expression Using Agroinfiltration To Study Polyadenylation In Plants
Carol Von Lanken and Arthur G. Hunt
12. A 3' RACE Protocol To Confirm Polyadenylation Sites
Liuyin Ma and Arthur G. Hunt
Part III: Genome-Scale Study of Polyadeylation in Plants
13. Phage Display Library Screening for Identification of Interacting Protein Partners
Balasubrahmanyam Addepalli, Suryadevara Rao, and Arthur G. Hunt
14. Genome-wide Determination Of Poly(A) Site Choice In Plants
Pratap Kumar Pati, Liuyin Ma, and Arthur G. Hunt
15. DNA/RNA Hybrid Primer Mediated Poly(A) Tag Library
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Construction for Illumina Sequencing
Man Liu, Xiaohui Wu, and Qingshun Quinn Li
16. Poly(A) Tag Library Construction from 10 ng Total RNA
Jingyi Cao and Qingshun Q. Li
17. A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-specific RNA-Seq Libraries
Arthur G. Hunt
18. Genome-Wide Analysis Of Distribution of RNA Polymerase II isoforms using ChIP-seq
Laura de Lorenzo
Man Liu, Xiaohui Wu, and Qingshun Quinn Li
16. Poly(A) Tag Library Construction from 10 ng Total RNA
Jingyi Cao and Qingshun Q. Li
17. A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-specific RNA-Seq Libraries
Arthur G. Hunt
18. Genome-Wide Analysis Of Distribution of RNA Polymerase II isoforms using ChIP-seq
Laura de Lorenzo
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Bibliographische Angaben
- 2014, 2015, IX, 225 Seiten, 23 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Arthur G. Hunt, Qingshun Quinn Li
- Verlag: Springer, Berlin
- ISBN-10: 1493921746
- ISBN-13: 9781493921744
- Erscheinungsdatum: 09.12.2014
Sprache:
Englisch
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