RNA Imaging
Methods and Protocols
(Sprache: Englisch)
This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of...
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Klappentext zu „RNA Imaging “
This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of imaging methodologies, and their interpretation. An essential element in the development and optimization of these therapies is the ability to measure the bioavailability and functionality of the RNA/oligonucleotide molecule after administration into the body. Noninvasive imaging provides the necessary set of tools to accomplish this in authentic physiologic environments and across time. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, RNA Imaging: Methods and Protocols serves physicians, scientists, and graduate students who are either new to the field of RNA-based imaging and its associated therapeutic applications or who wish to be apprised of recent advances in the state of the art.
Inhaltsverzeichnis zu „RNA Imaging “
1. Imaging Functional Nucleic Acid Delivery to SkinRoger L. Kaspar, Robyn P. Hickerson, Emilio González-González, Manuel A. Flores, Tycho P. Speaker, Faye A. Rogers, Leonard M. Milstone, and Christopher H. Contag
2. In Vivo Magnetic Resonance Imaging of Small Interfering RNA Nanodelivery to Pancreatic Islets
Ping Wang and Anna Moore
3. Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy
Marie-France Penet, Zhihang Chen, Noriko Mori, Balaji Krishnamachary, and Zaver M. Bhujwalla
4. Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer
Wei Chen and Lily Yang
5. Analyses of Tumor Burden In Vivo and Metastasis Ex Vivo Using Luciferase-Expressing Cancer Cells in an Orthotopic Mouse Model of Neuroblastoma
Frances L. Byrne, Joshua A. McCarroll, and Maria Kavallaris
6. Indium-Labeling of siRNA for Small Animal SPECT Imaging
Steven Jones and Olivia Merkel
7. Imaging of Electrotransferred siRNA
Muriel Golzio and Justin Teissié
8. Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents
Julien A. Boos and Iwan Beuvink
9. siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo
Seung Koo Lee and Ching-Hsuan Tung
10. Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells
Amol Kavishwar and Zdravka Medarova
11. Molecular Beacon-Based MicroRNA Imaging During Neurogenesis
Jonghwan Lee and Soonhag Kim
12. Hypoxia-Responsive Copolymer for siRNA Delivery
Federico Perche, Swati Biswas, Niravkumar R. Patel, and Vladimir P. Torchilin
13. Controlling RNA Expression in Cancer Using Iron Oxide Nanoparticles Detectable by MRI and In Vivo Optical Imaging
Zdravka Medarova, Mustafa Balcioglu, and Mehmet V. Yigit
14. Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures
Karmele Valencia and Fernando
... mehr
Lecanda
15. Positive Bioluminescence Monitoring of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES
Patrick Baril and Chantal Pichon
16. MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery
Yoko Endo-Takahashi, Yoichi Negishi, Ryo Suzuki, Kazuo Maruyama, and Yukihiko Aramaki
15. Positive Bioluminescence Monitoring of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES
Patrick Baril and Chantal Pichon
16. MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery
Yoko Endo-Takahashi, Yoichi Negishi, Ryo Suzuki, Kazuo Maruyama, and Yukihiko Aramaki
... weniger
Bibliographische Angaben
- 2015, 1st ed. 2016, XI, 218 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Zdravka Medarova
- Verlag: Springer, Berlin
- ISBN-10: 1493931474
- ISBN-13: 9781493931477
Sprache:
Englisch
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