Structural Genomics
General Applications
(Sprache: Englisch)
The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research...
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Produktinformationen zu „Structural Genomics “
The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.
Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.
Klappentext zu „Structural Genomics “
The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.
Inhaltsverzeichnis zu „Structural Genomics “
Part I. Cloning, Expression and Protein Production1. DisMeta - a Meta Server for Construct Design and Optimization, Yuanpeng Janet Huang, Thomas B. Acton, and Gaetano T. Montelione
2. Stable Expression Clones and Auto-Induction for Protein Production in E. Coli, F. William Studier
3. High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli, Natalie J. Saez and Renaud Vincentelli
4. Medium Throughput Production of Recombinant Human Proteins: Ligation Independent Cloning, Claire Strain-Damerell, Nicola A. Burgess-Brown, Pravin Mahajan, and Opher Gileadi
5. Medium Throughput Production of Recombinant Human Proteins: Protein Production in E. coli, Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Opher Gileadi, and Susanne Gräslund
6. Medium Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells, Pravin Mahajan, Nicola A. Burgess-Brown, Claire Strain-Damerell, and Opher Gileadi
7. OmniBac: Universal Multigene Transfer Plasmids for Baculovirus Expression Vector Systems, Deepak B. Thimiri Govinda Raj, Lakshmi S. Vijayachandran, and Imre Berger
8. Multiprotein Complex Production in Insect Cells by Using Polyproteins, Yan Nie, Itxaso Bellon-Echeverria, Simon Trowitzsch, Christoph Bieniossek, and Imre Berger
9. Expression Screening in Mammalian Suspension Cells, Susan D. Chapple and Michael R. Dyson
10. Cell-free Expression of Protein Complexes for Structural Biology Takaho Terada, Takeshi Murata, Mikako Shirouzu, and Shigeyuki Yokoyama
11. Cell-Free Protein Synthesis for Functional and Structural Studies, Shin-ichi Makino, Emily T. Beebe, John L. Markley, and Brian G. Fox
12. Insoluble Protein Purification with Sarkosyl: Facts and Precautions, Ben Chisnall, Courtney Johnson, Yavuz Kulaberoglu, and Yu Wai Chen
Part II. Experimental Structure Determination and Characterisation
13. Estimation of Crystallization Likelihood Through a Fluorimetric Thermal Stability Assay, Vincent
... mehr
Mariaule, Florine Dupeus, and José A. Márquez
14. CrystalDirect(TM): A Novel Approach for Automated Crystal Harvesting Based on Photoablation of Thin Films, Jose A. Marquez and Florent Cipriani
15. Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropy, Michael R. Sawaya
16. Applications of NMR-Based PRE and EPR-Based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure Determination, Yunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick, and Michael A. Kennedy
17. A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labelled Mutants for NMR Studies of High Molecular Weight Proteins, Elodie Crublet, Rime Kerfah, Guillaume Mas, Marjolaine Noirclerc-Savoye, Violaine Lantez, Thierry Vernet, and Jerome Boisbouvier
18. High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach, Kevin N. Dyer, Michal Hammel,, Robert P. Rambo, Susan E. Tsutakawa, Ivan Rodic, Scott Classen, John A. Tainer, and Greg L. Hura
19. Measuring Spatial Restraints on Native Protein Complexes using Isotope-Tagged Chemical Cross-Linking and Mass Spectrometry, Franz Herzog
Part III. Computational Methods and Structural Data Analyses
20. Modeling of Proteins and Their Assemblies with the Integrative Modeling Platform, Benjamin Webb, Keren Lasker, Javier Velázquez-Muriel, Dina Schneidman-Duhovny, Riccardo Pellarin, Massimiliano Bonomi, Charles Greenberg, Barak Raveh, Elina Tjioe, Daniel Russel, and Andrej Sali
21. The Quality and Validation of Structures from Structural Genomics, Marcin J. Domagalski, Heping Zheng, Matthew D. Zimmerman, Zbigniew Dauter, Alexander Wlodawer, and Wladek Minor
22. Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb, Andrei L. Turinsky, Sabry Razick, Brian Turner, Ian M. Donaldson, and Shoshana J. Wodak
23. Mespeus,, A Database of Metal Interactions with Proteins, Marjorie M. Harding and Kun-Yi Hsin
24. High-Quality Macromolecular Graphics on Mobile Devices: A Quick Starter's Guide, Chin-Pang Benny Yiu and Yu Wai Chen
14. CrystalDirect(TM): A Novel Approach for Automated Crystal Harvesting Based on Photoablation of Thin Films, Jose A. Marquez and Florent Cipriani
15. Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropy, Michael R. Sawaya
16. Applications of NMR-Based PRE and EPR-Based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure Determination, Yunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick, and Michael A. Kennedy
17. A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labelled Mutants for NMR Studies of High Molecular Weight Proteins, Elodie Crublet, Rime Kerfah, Guillaume Mas, Marjolaine Noirclerc-Savoye, Violaine Lantez, Thierry Vernet, and Jerome Boisbouvier
18. High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach, Kevin N. Dyer, Michal Hammel,, Robert P. Rambo, Susan E. Tsutakawa, Ivan Rodic, Scott Classen, John A. Tainer, and Greg L. Hura
19. Measuring Spatial Restraints on Native Protein Complexes using Isotope-Tagged Chemical Cross-Linking and Mass Spectrometry, Franz Herzog
Part III. Computational Methods and Structural Data Analyses
20. Modeling of Proteins and Their Assemblies with the Integrative Modeling Platform, Benjamin Webb, Keren Lasker, Javier Velázquez-Muriel, Dina Schneidman-Duhovny, Riccardo Pellarin, Massimiliano Bonomi, Charles Greenberg, Barak Raveh, Elina Tjioe, Daniel Russel, and Andrej Sali
21. The Quality and Validation of Structures from Structural Genomics, Marcin J. Domagalski, Heping Zheng, Matthew D. Zimmerman, Zbigniew Dauter, Alexander Wlodawer, and Wladek Minor
22. Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb, Andrei L. Turinsky, Sabry Razick, Brian Turner, Ian M. Donaldson, and Shoshana J. Wodak
23. Mespeus,, A Database of Metal Interactions with Proteins, Marjorie M. Harding and Kun-Yi Hsin
24. High-Quality Macromolecular Graphics on Mobile Devices: A Quick Starter's Guide, Chin-Pang Benny Yiu and Yu Wai Chen
... weniger
Bibliographische Angaben
- 2013, Repr. d. Ausg. v. 2013, XV, 358 Seiten, 63 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Yu Wai Chen
- Verlag: Springer, Berlin
- ISBN-10: 1627036903
- ISBN-13: 9781627036900
- Erscheinungsdatum: 20.11.2013
Sprache:
Englisch
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